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KMID : 0525720150200040225
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Journal of Chitin and Chitosan
2015 Volume.20 No. 4 p.225 ~ p.228
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nfluence of Chitin Amendment into Liquid Culture Medium on Virulence of Entomopathogenic Fungus Beauveria bassiana
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Jeong Ga-Young
Yoon Jin-Ju
Kim Jeong-Jun
Han Ji-Hee
Lee Sang-Yeob
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Abstract
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Cotton aphid is one of the most destructive insect pests in greenhouse, especially cultivating cucumber, pepper and watermelon. As alternative pest control agents the importance of entomopathogenic fungi is increasing. However, usage of mycopesticides are not increasing we have been expecting because of slow and fluctuating mortality. Therefore, in this study we conducted bioassays to select media of a Beauveria bassiana isolate which can produce spores or culture filtrate of high virulence against 3rd instar of Aphis gossypii when chitin or oil was added in media. The culture filtrates and culture fluids from media amended with colloidal chitin or oil showed lower mortality of the aphid than culture filtrate or culture fluid without chitin or oil. Control efficacy of culture fluids or culture filtrate was highest with AD medium compared with other media.
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KEYWORD
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Beauveria bassiana, Chitin, Cotton aphid, Culture fluid, Culture filtrate
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